Plate Coating

In cellular assay it is often advantageous to use coated microtiter plates in order to enhance cell attachment to the surface of the containment. Typical protocols require a short incubation time plus washing and drying of the plates. Blue®Bench is the perfect set-up for poly-L-lysine, collagen or other coating protocols because dispensing / incubation / evacuation are easily implemented by custom programs for a walk away workflow. Specifically in 1536 well format it is required to generate identically coated plates - ready to use.

Workflow

Empty Plate

01

Dispense Buffer

02

Evacuate Buffer

03

Dispense Coating Solution

04

Incubate

05

Evacuate Coating Solution

06

Dispense Blocking Solution

07

Incubate

08

Evacuate Blocking Solution

09

Ready To Use

10

Application Notes

19. August 2024

HLA microarray production using Blue®Washer

Blue®Washer Optimizes HLA Microarray Production with High Spot Integrity

The Authors evaluated the Blue®Washer for the production of HLA microarrays, demonstrating its compatibility by "maintaining spot integrity and sensitivity at low (35-50g) g-forces" during washes. Blue®Washer accelerates and simplifies the manufacturing process, making it suitable for "high-throughput production while preserving the quality" of the microarrays.

Download Paper

6. September 2021

Plate Coating Protocol

Knight al al. (2018) desribe  a coating protocol for 1536 MTPs using BlueWasher and achieve ready to go plates for cellular assays. Using a dynamic mass distribution (DMR) assay they could show an improvement of z' - value of about 1.0 due to removal of resdual fibronectin with the BlueWasher.

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Each assay is different! Let us know about your specific workflows & individual targets to get a customized Blue®Washer configuration for your assay campaigns.
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2D Cellular Assays
3D Cellular Assays
Suspension Cells
Cell Seeding
Magnetic Bead Assays*
Genomics - NGS Assays*
Dispensing
Plate Coating
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