ACS Chemical Biology

2021 Paper

Blue® Washer used for quick and easy washing of cells (6 wash steps) in the NanoClick assay

Authors sought to develop a tool to measure uptake of peptide molecules that enter the cell via different mechanisms. NanoClick is a High Throughput, Target-Agnostic Peptide Cell Permeability assay built on three underlying technologies—in-cell copper-free Click chemistry and Promega’s HaloTag® and NanoBRET™. The Blue®Washer was employed for cell washing steps, removal of DIBAC-CA solution and the dye.

AstraZeneca

2016 Paper

Blue® Washer enabling 1536w High Throughput (HT) cell based screening 

Sinead Knight et. al. (AZ, Cambridge, UK) describes how the Blue® Washer allowed miniaturizing a range of cell assays into 1536w format. Improved data quality and reduced number of wash steps in 384w assays shown. Cell assays currently limited in throughput due to cost and complex protocols may be enabled.

AstraZeneca

2017 SLAS Webinar

Helen Plant (AZ, Alderley Park, UK) disusses how the Blue® Washerintegrates centrifugal emptying with the individual addition of up to four separate solutions for complex phenotypic assays. "Successful conversion of complex phenotypic assays to 1536w results in up to a 50 percent reduction in cells needed & 4x time reduction for full HTS screening campaign".

Bristol-Myers Squibb

2017 Paper

Blue® Washer removing medium from iNs cells

Tao Wang et. al. (BMS, Hopewell, NJ) in their paper "Measurement of beta-Arrestin Recruitment for GPCR Targets" use the Blue®Washer to wash cells in their Sample Protocol for Antagonist Assays. This paper has been included in the 2018 edition of the Assay Guidance Manual (Eli Lilly & Company and the National Center for Advancing Translational Sciences, Bethesda, MD)

Merck

2017 Paper

Blue® Washer used for media removal at Merck Kenilworth

Corin Miller et. al. (Merck & Co, Kenilworth, NJ) in their paper "GPR40 partial agonist MK-2305 lower fasting glucose in the Goto Kakizaki rat via suppression of endogenous glucose production" use Blue® Washer to remove growth media in in-vitro receptor potency assay.

AstraZeneca

SLAS2017 presentation

Blue® Washer integrated into AstraZeneca's fully automated 1536w HCS platform

Paul Harper (AstraZeneca, Alderley Park, UK) integrated Blue® Washers into their fully automated HighRes Cellario and Agilent VWorks platforms, replacing Tecan PW384 and Biotek 405 aspiration based washers.

Merck

SLAS2017 presentation

Cell-Based In Vitro Assay Automation:  Avoid false negatives with Blue® Washer

BAdam Weinglass and Mary Jo Wildey (Merck & Co, Kenilworth, NJ) show how Blue® Washer  improves data consistency and decreases rate of false negatives.  Historically inactive compounds aligned when Blue® Washer was used for media removal

SCRIPPS Florida

2017 Paper

Blue® Washer delivering high z' for 1536w drug library screen

Brande Thomas-Fowlkes et. al. (Merck & Co, Kenilworth, NJ) in their paper "Cell-Based In Vitro Automation: Balancing Shurong Hou et. al. (Dept of Molecular Therapeutics, Scripps Research Institute, Juniper, FL) in their paper "Drug Library Screening for the Identification of Ionophores That Correct the Mistrafficking Disorder Associated with Oxalosis Kidney Disease" use Blue®Washer for 4 cell wash steps in a 1536w HCS, reporting Z′ 0.70 ± 0.07.

AstraZeneca

2018 Poster: Bomi et al.

Blue® Washer dramatically shortens wash time for intracellular drug distribution at AZ

Bomi Jung et. al. (AZ, Gothenburg SWE) in their ELRIG poster "High density assessment of drug distribution profiles in cultured cells" reduced wash time from 20-30 minutes to <1min using the Blue® Washer.

Scripps Research Institute

2019 Paper: Sridharan et. al

Blue® Washer removing medium from iNs cells

Banu Priya Sridharan et. al. (The Scripps Research Molecular Screening Center, Juniper, FL) in their paper "A Simple Procedure for Creating Scalable Phenotypic Screeing Assays in Human Neurons" describe using Blue® Washer's GentleSpin cycle to remove growth medium from IPSC-derived human neurons (iNs) in a 384w format for subsequent HCS imaging

Astra Zeneca

SLAS2019 presentation

AstraZeneca and BlueCatBio collaborate on Blue® Washer assay optimization

Helen Plant (AstraZeneca, Alderley Park, UK) and BlueCatBio collaborated to develop a see-through  rotor cover, allowing observation of washes in progress to optimize Blue® Washer assay protocols.

Recursion

2020 Paper

Blue® Washer helped generate great, conclusive data with 1536w screen, incredibly fast

Katie Heiser, Peter F. McLean, et al (Recursion, SLC, UT) in their paper "Identification of potential treatments for COVID-19 through AI-enabled phenomic analysis of human cells infected with SARS-CoV-2" used their Blue® Washer for all staining and imaging washes and reagent additions achieving z' of >0.8. 

Merck

2020 Paper

Blue® Washer reduces "false negatives", improves GPCR assay performance & reproducibility

Brande Thomas-Fowlkes et. al. (Merck & Co, Kenilworth, NJ) in their paper "Cell-Based In Vitro Automation: Balancing Technology and Data Reproducibility/ Predictability" show how Blue® Washer reduces "false negatives",improves assay quality and reduces the replicates in SAR assay support for Merck HTRF and IP-I cell-based, functional adherent 384w assays.