Blue®Washer enabling 1536w HTS, improving z'

Helen Plant (AstraZeneca, Alderley Park, UK) shows how the Blue®Washer enabled 384w to 1536w conversion of complex, high-content phenotypic high-throughput screens, and in-house fibronectin coating of 1536w plates, resulting in up to a 25% reduction in cells needed, ~4x time reduction, economical screening of expensive assays.


Blue®Washer integrated into AstraZeneca's fully automated 1536w HCS platform

Paul Harper (AstraZeneca, Alderley Park, UK) integrated Blue®Washers into their fully automated HighRes Cellario and Agilent VWorks platforms, replacing Tecan PW384 and Biotek 405 aspiration based washers.

2016 paper

2017 paper

SLAS2017 presentation

SLAS2016 presentation

Blue®Washer delivering high z' for 1536w drug library screen

Shurong Hou et. al. at the Dept of Molecular Therapeutics, Scripps Research Institute, Juniper, FL, in their paper "Drug Library Screening for the Identification of Ionophores That Correct the Mistrafficking Disorder Associated with Oxalosis Kidney Disease" use Blue®Washer for 4 cell wash steps in a 1536w HCS, reporting Z′ 0.70 ± 0.07.

Blue®Washer used for media removal at Merck Kenilworth

Corin Miller et. al. at Merck, Kenilworth, NJ, in their paper "GPR40 partial agonist MK-2305 lower fasting glucose in the Goto Kakizaki rat via suppression of endogenous glucose production" use Blue®Washer to remove growth media in in-vitro receptor potency assay.

2017 paper

2017 paper

Astra Zeneca


Cellular Assays: Download literature

Merck


Blue®Washer used for cell wash at BMS

Tao Wang et. al. at BMS, Hopewell, NJ, in their paper "Measurement of beta-Arrestin Recruitment for GPCR Targets" use the Blue®Washer to wash cells in their Sample Protocol for Antagonist Assays. This paper has been included in the 2018 edition of the Assay Guidance Manual (Eli Lilly & Company and the National Center for Advancing Translational Sciences, Bethesda, MD)

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AstraZeneca and BlueCatBio collaborate on BlueWasher assay optimization

Helen Plant (AstraZeneca, Alderley Park, UK) and BlueCatBio collaborated to develop a see-through  rotor cover, allowing observation of washes in progress to optimize Blue®Washer assay protocols.

Blue®Washer dramatically shortens wash time for intracellular drug distribution at AZ

Bomi Jung et. al. at AZ, Gothenburg SWE, in their ELRIG poster "High density assessment of drug distribution profiles in cultured cells" reduced wash time from 20-30 minutes to <1min using the Blue®Washer.

SLAS2017 presentation

SCRIPPS Florida


Cell-Based In Vitro Assay Automation:  Avoid false negatives with Blue®Washer

Adam Weinglass and Mary Jo Wildey (Merck & Co, Kenilworth, NJ) show how Blue®Washer  improves data consistency and decreases rate of false negatives.  Historically inactive compounds aligned when Blue®Washer was used for media removal.

Blue®Washer enabling 1536w High Throughput (HT) cell based screening

Sinead Knight et. al. (AZ, Cambridge, UK) describes how the Blue®Washer allowed miniaturizing a range of cell assays into 1536w format. Improved data quality and reduced number of wash steps in 384w assays shown. Cell assays currently limited in throughput due to cost and complex protocols may be enabled.

 2018 poster

2019 paper

SLAS2019 presentation

(for literature on 3D cellular screens, go to Magnetic Assays)

BMS


Blue®Washer removing medium from iNs cells

Banu Priya Sridharan et. al. at The Scripps Research Molecular Screening Center, Juniper, FL, in their paper "A Simple Procedure for Creating Scalable Phenotypic Screeing Assays in Human Neurons" describe using Blue®Washer's GentleSpin cycle to remove growth medium from IPSC-derived human neurons (iNs) in a 384w format for subsequent HCS imaging.