Blue®Washer enabling 1536w HTS, improving z'
Helen Plant (AstraZeneca, Alderley Park, UK) shows how the Blue®Washer enabled 384w to 1536w conversion of complex, high-content phenotypic high-throughput screens, and in-house fibronectin coating of 1536w plates, resulting in up to a 25% reduction in cells needed, ~4x time reduction, economical screening of expensive assays.
Blue®Washer integrated into AstraZeneca's fully automated 1536w HCS platform
Paul Harper (AstraZeneca, Alderley Park, UK) integrated Blue®Washers into their fully automated HighRes Cellario and Agilent VWorks platforms, replacing Tecan PW384 and Biotek 405 aspiration based washers.
Blue®Washer enabling 1536w High Throughput (HT) cell based screening
Sinead Knight et. al. (AZ, Cambridge, UK) describes how the Blue®Washer allowed miniaturizing a range of cell assays into 1536w format. Improved data quality and reduced number of wash steps in 384w assays shown. Cell assays currently limited in throughput due to cost and complex protocols may be enabled.
Cellular Assays: Download literature
(for literature on 3D cellular screens, go to Magnetic Assays)
Blue®Washer used for cell wash at BMS
Tao Wang et. al. at BMS, Hopewell, NJ, in their paper "Measurement of beta-Arrestin Recruitment for GPCR Targets" use the Blue®Washer to wash cells in their Sample Protocol for Antagonist Assays. This paper has been included in the 2018 edition of the Assay Guidance Manual (Eli Lilly & Company and the National Center for Advancing Translational Sciences, Bethesda, MD)
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Blue®Washer delivering high z' for 1536w drug library screen
Shurong Hou et. al. at the Dept of Molecular Therapeutics, Scripps Research Institute, Juniper, FL, in their paper "Drug Library Screening for the Identification of Ionophores That Correct the Mistrafficking Disorder Associated with Oxalosis Kidney Disease" use Blue®Washer for 4 cell wash steps in a 1536w HCS, reporting Z′ 0.70 ± 0.07.
Blue®Washer used for media removal at Merck Kenilworth
Corin Miller et. al. at Merck, Kenilworth, NJ, in their paper "GPR40 partial agonist MK-2305 lower fasting glucose in the Goto Kakizaki rat via suppression of endogenous glucose production" use Blue®Washer to remove growth media in in-vitro receptor potency assay.
Blue®Washer removing medium from iNs cells
Banu Priya Sridharan et. al. at The Scripps Research Molecular Screening Center, Juniper, FL, in their paper "A Simple Procedure for Creating Scalable Phenotypic Screeing Assays in Human Neurons" describe using Blue®Washer's GentleSpin cycle to remove growth medium from IPSC-derived human neurons (iNs) in a 384w format for subsequent HCS imaging.
AstraZeneca and BlueCatBio collaborate on BlueWasher assay optimization
Helen Plant (AstraZeneca, Alderley Park, UK) and BlueCatBio collaborated to develop a see-through rotor cover, allowing observation of washes in progress to optimize Blue®Washer assay protocols.
Cell-Based In Vitro Assay Automation: Avoid false negatives with Blue®Washer
Adam Weinglass and Mary Jo Wildey (Merck & Co, Kenilworth, NJ) show how Blue®Washer improves data consistency and decreases rate of false negatives. Historically inactive compounds aligned when Blue®Washer was used for media removal.