Blue®Washer / Blue®Washer XL

The centrifugal evacuation technology for all MTPs

Clean data, shorter turnaround time, reduced cost for your assay campaigns

The Blue®Washer uses contact-free centrifugation technology to remove liquids from 96, 384 and 1536 microwell plates without disposable tips. This environmentally friendly technology allows you to handle cellular, bead based, genomic, and ELISA assays contamination free with no residual volume, eliminating background and variability in assay data. The integrated dispenser leads to unparalleled efficiency in your assay campaigns.

Drive your applications with the centrifugal Blue®Washer

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Features

Centrifugation

Blue®Washer uses centrifugation instead of aspiration to remove liquids from microtiter plates. With Blue®Washer's centrifugal technology, microtiter plates are spun around a horizontal axis, and liquids are removed from wells by the centrifugal forces, immediately leaving the rotor chamber through Blue®Washer's patented passive drain. Centrifugal evacuation significantly reduces residual volumes and variability in media changes or when removing supernatant. Lower background and variability mean better, more reproducible data from cellular screens. For magnetic bead* assays, Blue®Washer eliminates the need for disposable tips, minimizes bead-loss and dramatically reduces turn-around time.

Liquid Inputs

In the standard configuration, liquids from up to seven inputs can be dispensed through a shared dispenser head and internal liquid path. Each liquid input is adressed individually and tuned with the Blue®Flow software.

Shared Dispenser

Slanted or straight dispenser heads are available for 6, 12, 24, 48, 96, 384 and 1536 well plates. The Blue®Flow graphical user interface allows selection of cell-friendly dispense modes to minimize loss of weakly adherent/suspension cells.

Dedicated Dispenser

In the dedicated dispenser configuration, up to 3 dedicated liquid inputs are added on the right side of the Blue®Washer. Each input uses its own “dedicated” dispenser head and internal liquid path. Eliminating the need to purge a shared liquid path when switching liquids makes it economical to dispense precious reagents. In addition, different dispenser heads can be mixed and matched eliminating the need to swap dispensers when switching plate types.

Drum Clean

The internal rotor chamber and all dispenser components – shared and dedicated – are automatically cleaned and disinfected using Blue®Washer’s patent pending self-cleaning methods. Blue®Washer’s submersive DrumClean method reliably removes any built-up deposits from inside the rotor and rotor chamber for periodic, intense decontamination.
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Linear Drive

Blue®Washer’s patented linear drive positions plates with micrometer precision during dispensing, ensuring dispense jets enter wells in the exact location required by the application, every time. For instance, the dispense jet can be trained to hit the front wall of the wells to ensure that sensitive cells do not detach.

Plate Formats

Blue®Washer can handle any SBS format plate up to 17.4mm height including 1536 well strips or slides. Blue®Washer XL is designed for taller plates up to 27mm height in applications such as 3D cell-based assays or higher volume magnetic bead assays*. To retain magnetic beads while spinning, various magnetic carriers are available in 96 and 384 formats covering a wide variety of plate specifications.

Blue® Flow 
Graphical User Interface

Intuitive Graphical User Interface featuring, pre-set application-specific evacuation methods along with a click-and-drag method manager to customize protocols easily.

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Specifications

Blue®Washer
Blue®Washer XL
Applications
Contactless media change & liquid evacuation with near zero, reproducible residual volume Magnetic bead* washes with magnetic carrier for 96, 384, 1536 well MTPs
Contactless media change & liquid evacuation with near zero, reproducible residual volume Magnetic bead* washes with magnetic carrier for 6, 12, 24, 48, 96 and 384 well MTPs
Compatibility
Any SBS format plate up to 17.4mm height (including 1536 well) strip or slides, custom formats programmable; dispensing into 96 and 384 well plates; 1536 well MTP option
Any SBS format plate up to 27mm height strip or slides, custom formats programmable; dispensing into 6, 12, 24, 48, 96 and 384 well plates
Centrifuge system
Liquid evacuation by centrifugation; plates positioned in symmetric rotor with well openings facing outwards to expel liquids. Rotor driven by computer controlled, high power servo motor
Residual volume
0.01μl to 5μl per well, depending on application-specific evacuation method selected
Shaking & incubation
Programmable speeds, rocking angles and time, ideal to break up liquid layers
Centrifuge specs
RPM: 3 to 3,500 RPM (= 630 g), Acceleration: <1,500 RPM/s, Deceleration:< 1,000 RPM/s
Maximum weight difference between active plate and balance plate in dry condition:
15 g @ 3,500 rpm, 20 g @ 2,000 rpm, 35 g @ 1,500 rpm, 70 g @ 1,000 rpm
Dispense system
Precision membrane pump and electromechanical valves controlled by on-board Linux CPU
Liquid inputs
Choice of 4 or 7 liquid inputs with Luer lock fittings
Specifications
(all volumes per well)
Manifold: 8, 16, or 32 needles (for 96/384/1536 well MTP)
Dispense jet position: Slanted dispenser mount to aim at well walls, plate off-set adjustable
Dispense pressure: adjustable in 5 steps, from approx. 25% to full pressure
Dispense speeds: 96w MTP: 7 sec @ 100μl; 384w MTP: 13 sec @ 50μl; 1536w MTP: 13 sec @ 8μl
Range & accuracy: 96 and 384 MTP: >1μl, < 3%CV @ 100μl; 1536 MTP: >3μl, < 5%CV @ 10μl
Manifold: 2, 3,4, 6, 8, 16 needles (for 6/12/24/48/ 96/384well MTP)
Dispense jet position: Slanted dispenser mount to aim at well walls, plate off-set adjustable
Dispense pressure: adjustable in 5 steps, from approx. 25% to full pressure
Dispense speeds: 96w MTP: 7 sec @ 100μl; 384w MTP: 13 sec @ 50μl
Range & accuracy: 6, 12, 24, 48 MTP: >1μl; 96 and 384 MTP: >5μl, < 3%CV @ 100μl;
Internal dead volume
8 ml (input connector to dispenser pin tips)
Tubes
Reagent tubes: length: 1m, Ø outside: 5mm, Ø inside: 3mm; Luer locks on both ends connect Blue®Washer input to Luer lock on GL-45 cap for reagent bottle, color-coded
Software and operation
PC-based GUI with quick access to pre-set, application specific evacuation methods and editor to customize methods and program workflows. Autonomous mode to run methods from Blue®Washer’s internal memory
Automation
Easy plate load/ pick-up by simple robotic gripper; standard API, USB and Ethernet
Available automated plate loading via Blue®Bench; choice of plate storage in up to 5 stacks or random access hotels with auto de-lidding/ re-lidding
Safety
Centrifugation shut off in case of excessive vibration; sensor controlled interlocking door
Cleaning & decontamination
Single touch QuickClean and IntenseClean protocols with custom Blue®Daily and Blue®Intense cleaning solutions highly effective to clean and decontaminate Blue®Washer interior
Dimensions (HxWxD)
20.5 cm x 36.5cm x 69.0 cm | 8.1" x 14.4" x 27.2"
20.5 cm x 36.5cm x 69.8 cm | 8.1" x 14.4" x 27.5"
electrical and shared liquid connections on left side wall, dedicated liquid connections on right side wall
Weight
27 kg | 60 lbs
28 kg | 62 lbs
Power requirements
100 – 240 V, 50/60 Hz, 350 W, auto sensing, I max = 10A
Ambient temperature
15°C – 55°C | 60 F – 130F

Blue®Plate

Blue®Plate enables fully automated, high-throughput cell washes for flow cytometry
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Carrier + Balance

Set of 2 carriers with 52mm cut-out compatible with HRB grippers
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Blue® Daily
Cleaning solution for the daily cleaning procedure of rotor and rotor chamber
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Blue® Intense
Cleaning solution to avoid corrosion and extend the lifetime of rotor and rotor chamber
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Test your customized Blue®Washer configuration

Each assay is different! Let us know about your specific workflows & individual targets to get a customized Blue®Washer configuration for your assay campaigns.
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2D Cellular Assays
3D Cellular Assays
Suspension Cells
Cell Seeding
Magnetic Bead Assays*
Genomics - NGS Assays*
Dispensing
Plate Coating
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