BlueWasher enabling 1536w HTS, improving z'
Helen Plant (AstraZeneca, Alderley Park, UK) in this SLAS2016 presentation, shows how the BlueWasher enabled conversion of complex, high-content phenotypic high-throughput screens from 384w to 1536w plates, as well as in-house coating of 1536w plates with fibronectin. Near zero residual volume by using centrifugation rather than aspiration is enabling for 1536w formats. Successful conversion to 1536w format resulted in up to a 25% reduction in cells needed and ~4x time reduction to perform a full HTS screening campaign. It has also allowed economical screening of expensive assay formats and cell types in high-content phenotypic HTS screens.
BlueWasher used for media removal at Merck Kenilworth
Corin Miller et. al. at Merck, Kenilworth, NJ, in their paper "GPR40 partial agonist MK-2305 lower fasting glucose in the Goto Kakizaki rat via suppression of endogenous glucose production" describe using BlueWasher to remove growth media in their in-vitro receptor potency assay.
BlueWasher Dedicated Dispenser flyer
The BlueWasher can now be configured with up to 3 dedicated dispensers, in addition to the standard, shared dispenser, which can dispense from 4 or 7 inputs. This option makes it economical to dispense precious reagents into larger batches of plates - no interim flushing needed.
Cover Story pharma Tech Outlook:
The BlueWasher, one of pharma Tech Outlook's top 10 Drug Discovery innovations in 2017, is featuring in this cover story. Based on interviews and literature review, it explains why BlueWasher has quickly become the new standard for media change in cellular assays.
Flyer & Specs
Contamination-free plate evacuation wtih BlueWasher
This Technical Note presents data in support of the absence of cross-contamination when evacuating a mircoplate with the BlueWasher.
BlueWasher integrated into AstraZeneca's fully automated 1536w HCS platform
Paul Harper (AstraZeneca, Alderley Park, UK) in this SLAS2017 presentation, shows how they integrated BlueWashers into their fully automated HighRes Cellario and Agilent VWorks platforms, replacing Tecan PW384 and Biotek 405 aspiration based washers.
BlueWasher CAD files for system integration
3 dimensional CAD files (.STEP format) of the BlueWasher envelopes for hardware integration in automated solutions.
Note: downloaded file will show as random code of letters and numbers without extension. You may need to edit the file name and add the .STEP extension to view or import into your CAD program
BlueWasher delivering high z' for 1536w drug library screen at SCRIPPS Florida
Shurong Hou et. al. at the Department of Molecular Therapeutics, Scripps Research Institute, Juniper, FL, in their paper "Drug Library Screening for the Identification of Ionophores That Correct the Mistrafficking Disorder Associated with Oxalosis Kidney Disease" describe using BlueWasher for 4 cell wash steps in a miniaturized 1536w high-content assay, reporting a Z′ averaging 0.70 ± 0.07.
Cell-Based In Vitro Assay Automation: Fewer false negatives with BlueWasher
Adam Weinglass and Mary Jo Wildey (Merck & Co, Kenilworth, NJ) present different ‘technology’ options (Dump/Pat; 384W Plate Washers; Non-contact Centrifugation BlueWasher) for removing assay media prior to compound addition & determination of IP1 accumulation. BlueWasher results showed to be closer to "intrinsic, serum-free compound potency, improve data consistency and decrease the rate of false negatives." Show example of historically relatively inactive compounds that aligned when BlueWasher was used for media removal.
Using centrifugation instead of aspiration to remove liquids from microplates, BlueWasher reduces residual volumes 10x vs conventional plate washers, reducing background and variability, improving z' for typical adherent cell-based assays by 0.1 to 0.3.
Screen Design and Assay Technology SIG
The BlueBench solution brings the benefits of collaborative robots to scientists’ (cramped) benchtops. Flyer includes specifications and components.
The BlueWasher operates without a waste pump.
BlueWasher used for cell wash at BMS
Tao Wang et. al. at BMS, Hopewell, NJ, in their paper "Measurement of beta-Arrestin Recruitment for GPCR Targets" use the BlueWasher to wash cells in their Sample Protocol for Antagonist Assays. This paper has been included in the 2018 edition of the Assay Guidance Manual (Eli Lilly & Company and the National Center for Advancing Translational Sciences, Bethesda, MD)
BlueWasher and n3D enabling automation-friendly 3D cell screen
Shurong Hou (SCRIPPS, Jupiter, FL), in this SLAS2018 poster, shows how the n3D cell magnetization technology using the BlueWasher for media change and wash retains magnetized cells, allows for effective media evacuation and produces high quality results.
AZ ELRIG London 2018 poster
Copyright @ BlueCatBio. All rights reserved.
BlueWasher enabling 1536w High Throughput (HT) cell based screening
Major hurdles to adopting cell based assays for HT screening are the cost, availability of cells, and complex protocols. Miniaturization to 1536w format can reduce costs and increase throughput but brings major challenges in liquid handling for high-content assays requiring washing steps and coating of plates. Paper describes the novel application of the BlueWasher to facilitate the miniaturization of a range of 1536w cell assays and techniques to reduce edge effects, improving throughput and data quality. Cell assays currently limited in throughput because of cost and complex protocols may be enabled by the techniques presented in this study.
Download flyers, presentations, papers and CAD files for BlueCatBio products.
AstraZeneca and BlueCatBio collaborate on BlueWasher assay optimization
Helen Plant (AstraZeneca, Alderley Park, UK) in this SLAS2019presentation, shows how AZ and BlueCatBio collaborated to develop a see-through version of the rotor cover, allowing observation of washes in progress to optimize BlueWasher assay protocols.