BlueWasher may only be operated without wast pump in the area covered by the European Patent Office

BlueWasher integrated into AstraZeneca's fully automated 1536w HCS platform

Paul Harper (AstraZeneca, Alderley Park, UK) in this SLAS2017 presentation, shows how they integrated BlueWashers into their fully automated HighRes Cellario and Agilent VWorks platforms, replacing Tecan PW384 and Biotek 405 aspiration based washers.

Merck SLAS2017:

Screen Design and Assay Technology SIG

BlueBench flyer

The  BlueBench solution brings the benefits of collaborative robots to scientists’ (cramped) benchtops. Flyer includes specifications and components.

BlueWasher enabling 1536w HTS, improving z'

Helen Plant (AstraZeneca, Alderley Park, UK) in this SLAS2016 presentation, shows how the BlueWasher enabled conversion of complex, high-content phenotypic high-throughput screens from 384w to 1536w plates, as well as in-house coating of 1536w plates with fibronectin. Near zero residual volume by using centrifugation rather than aspiration is enabling for 1536w formats. Successful conversion to 1536w format resulted in up to a 25% reduction in cells needed and ~4x time reduction to perform a full HTS screening campaign. It has also allowed economical screening of expensive assay formats and cell types in high-content phenotypic HTS screens.

AstraZeneca SLAS2016

BlueWasher enabling 1536w High Throughput (HT) cell based screening

Major hurdles to adopting cell based assays for HT screening are the cost, availability of cells, and complex protocols. Miniaturization to 1536w format  can reduce costs and increase throughput but brings major challenges in liquid handling for high-content assays requiring washing steps and coating of plates. Paper describes the novel application of the BlueWasher to facilitate the miniaturization of a range of 1536w cell assays and techniques to reduce edge effects, improving throughput and data quality. Cell assays currently limited in throughput because of cost and complex protocols may be enabled by the techniques presented in this study.

BlueWasher delivering high z' for 1536w drug library screen at SCRIPPS Florida

Shurong Hou et. al. at the Department of Molecular Therapeutics, Scripps Research Institute, Juniper, FL, in their paper "Drug Library Screening for the Identification of Ionophores That Correct the Mistrafficking Disorder Associated with Oxalosis Kidney Disease" describe using BlueWasher for 4 cell wash steps in a miniaturized 1536w high-content assay, reporting a Z′ averaging 0.70 ± 0.07.

AstraZeneca SLAS2017

SCRIPPS paper

AstraZeneca paper

Cell-Based In Vitro Assay Automation:  Reproducibility/Predictability

Adam Weinglass and Mary Jo Wildey (Merck & Co, Kenilworth, NJ) discuss different ‘technology’ options (Dump/Pat; 384W Plate Washers; Non-contact Centrifugation BlueWasher) for removing assay media prior to compound addition & determination of IP1 accumulation. Assay performance, data reproducibility & predictability in a Structure Activity Relationship (SAR) workflow were impacted. Review of underlying factors leading to the selection and successful use of semi-automated/ automated tools.

BlueWasher CAD files for system integration

3 dimensional CAD files (.STEP format) of the BlueWasher envelopes for hardware integration in automated solutions. Choose BlueWasher2016 (or legacy BlueWasher which is 105mm less deep than BlueWasher2016).

Note: downloaded file will show as random code of letters and numbers without extension.  You may need to edit the file name and add the .STEP extension to view or import into your CAD program

CAD files

BlueWasher flyer

Using centrifugation instead of aspiration to remove liquids from microplates, BlueWasher achieves residual volumes 10x lower than conventional plate washers, reducing background and improving z' for typical adherent cell-based assays by 0.1 to 0.3.  Flyer includes specifications and descritption of application-specific evacuation settings.

Reproducible and adjustable post plate wash residual volumes down to 0.01µl/well

BlueWasher allows adjusting post wash residual volumes from 5µl to <0.01µl/well. BlueWasher is ideally suited to workflows with washing steps where precise, reproducible residual volumes are critical for high quality results.

Download flyers, presentations, papers and CAD files for BlueCatBio products.

Technical Note

Flyer & Specs

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