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Download presentation "Addressing the challenges of 1536-well cell based screening"given by AstraZeneca at SLAS2016 

Simpler Assay Design

Centrifugation mimicks "dump and tap" - simple automation of manual SOPs

  • Typical z' gain: 0.1 - 0.3
  • 10x lower residual volume reduces assay background and variability
  • Cleaner images
  • No cell loss from aspiration needles
  • Homogeneous evacuation
  • No assay data lost to clogged cannulas or warped plates
  • No need to re-calibrate needle positions
  • Residual volumes constant over time

Our BlueWasher's award-winning ability to improve z' for adherent cellular assays makes it the most cost-effective tool to boost drug discovery productivity.   With residual volumes 10x lower than conventional, tip-based plate washers, BlueWasher reduces assay background and variability, allowing imagers to produce even better data.  Ideal for assay development, high-throughput screening (HTS), high-content screening (HCS), FLIPR┬«, or expression assays, including 1536w, as part of a fully automated system, or a stand-alone workstation.

Better Data,

More Productive Discovery

FLIPR ® is a registered trademark of Molecular Devices, LLC
Enabling 1536-Well High-Throughput Cell-Based Screening through the Application of Novel Centrifugal Plate Washing.
Knight S, Plant H, McWilliams L, Murray D, Dixon-Steele R, Varghese A, Harper P, Ramne A, McArdle P, Engberg S, Bennett N, Blackett C, Wigglesworth M.
Author information

Abstract
Cell-based assays have long been important within hit discovery paradigms; however, improving the disease relevance of the assay system can positively affect the translation of small-molecule drug discovery, especially if adopted in the initial hit identification assay. Consequently, there is an increasing need for disease-relevant assay systems capable of running at large scale, including the use of induced pluripotent stem cells and donor-derived primary cells. Major hurdles to adopting these assays for high-throughput screening are the cost, availability of cells, and complex protocols. Miniaturization of such assays to 1536-well format is an approach that can reduce costs and increase throughput. Adaptation of these complex cell assays to 1536-well format brings major challenges in liquid handling for high-content assays requiring washing steps and coating of plates. In addition, problematic edge effects and reduced assay quality are frequently encountered. In this study, we describe the novel application of a centrifugal plate washer to facilitate miniaturization of a range of 1536-well cell assays and techniques to reduce edge effects, all of which improved throughput and data quality. Cell assays currently limited in throughput because of cost and complex protocols may be enabled by the techniques presented in this study.

KEYWORDS:
1536-well; cell assays; high-throughput; miniaturization; phenotypic screening

PMID:28027450, DOI:10.1177/2472555216683650
  • <30sec to evacuate any plate format, including 1536 well
  • 40cm x 70cm footprint
  • No need for compressed air or vacuum

High Throughput, Small Footprint

Miss fewer active compounds

Reduce false positives

No clogged needles,

more reproducible data

Link to paper (see abstract below): "Enabling 1536-Well High-Throughput Cell-Based Screening through the Application of Novel Centrifugal Plate Washing." by Sinead Knight et. al., AstraZeneca 
Read our interview with High Content Review featuring the BlueWasher for high content cellular assays

The BlueWasher operates without a waste pump.

  • Low residual eliminates wash steps
  • Simpler automation of manual assays
  • Simpler transition to 1536w
  • No need for complex design of "squirt-squirt-read" assays

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